Shen, Ji; Qiao, Juan; Zhang, Xinya; Qi, Li published the artcile< Dual-stimuli-responsive porous polymer enzyme reactor for tuning enzymolysis efficiency>, COA of Formula: C7H5NO2, the main research area is porous polymer enzyme reactor enzymolysis efficiency; Capillary electrophoresis; D-amino acid oxidase; Dual-stimuli-response; Porous polymer membrane enzyme reactor; Tuning enzymolysis efficiency.
A strategy for preparing a dual-stimuli-responsive porous polymer membrane enzyme reactor (D-PPMER) is described, consisting of poly(styrene-maleic anhydride-N-isopropylacrylamide-acrylate-3′,3′-dimethyl-6-nitro-spiro[2H-1-benzopyran-2,2′-indoline]-1′-esterspiropyran ester) [P(S-M-N-SP)] and D-amino acid oxidase. Tunable control via “”on/off”” 365 nm UV light irradiation and temperature variation was used to change the membrane surface configuration and adjust the enzymolysis efficiency of the D-PPMER. A chiral capillary electrophoresis technique was developed for evaluation of the enzymic efficiency of D-PPMER with a Zn(II)-dipeptide complex as the chiral selector and D,L-serine as the substrate. Interestingly, the enzymic kinetic reaction rate of D-PPMER under UV irradiation at 36°C (9.2 x 10-2 mM·min-1) was 3.2-fold greater than that of the free enzyme (2.9 x 10-2 mM·min-1). This was because upon UV irradiation at high temperature, the P(SP) and P(N) moieties altered from a “”stretched”” to a “”curled”” state to encapsulate the enzyme in smaller cavities. The confinement effect of the cavities further improved the enzymic efficiency of the D-PPMER. This protocol highlights the outstanding potential of smart polymers, enables tunable control over the kinetic rates of stimuli-responsive enzyme reactors, and establishes a platform for adjusting enzymolysis efficiency using two different stimuli.
Microchimica Acta published new progress about Contact angle. 21725-69-9 belongs to class isoxazole, and the molecular formula is C7H5NO2, COA of Formula: C7H5NO2.
Referemce:
Isoxazole – Wikipedia,
Isoxazole | C3H3NO – PubChem