In-Silico Screening of Novel Synthesized Thienopyrimidines Targeting Fms Related Receptor Tyrosine Kinase-3 and Their In-Vitro Biological Evaluation was written by Elmongy, Elshaymaa I.;Altwaijry, Najla;Attallah, Nashwah G. M.;AlKahtani, Manal Mubarak;Henidi, Hanan Ali. And the article was included in Pharmaceuticals in 2022.Quality Control of 3-(tert-Butyl)isoxazol-5-amine This article mentions the following:
The present investigation describes the design strategy and synthesis of novel thienopyrimidine compounds in addition to their anticancer activity targeting tyrosine kinase FLT3 enzyme. The synthesized compounds were subjected to a cytotoxic study where compounds I and II showed the most potent cytotoxicity against HT-29, HepG-2, and MCF-7 cell lines reflected by their IC50 values for I (1.21 ± 0.34, 6.62 ± 0.7 and 7.2 ± 1.9μM), for II (0.85 ± 0.16, 9.11 ± 0.3 and 16.26 ± 2.3μM) and better than that of reference standard which recorded (1.4 ± 1.16, 13.915 ± 2.2, and 8.43 ± 0.5μM), resp. Compounds’ selectivity to malignant cells was determined using selectivity assay, interestingly, all the tested compounds demonstrated an excellent selectivity index (SI) range from 20.2 to 99.7. Mol. docking studies were performed on the prepared compounds which showed promising binding affinity for FLT3 kinase enzyme and the main interactions between the synthesized ligands and kinase active site were similar to those between the co-crystallized ligand and the receptor. Further biol. exploration was performed using in-vitro FLT3 kinase enzyme inhibition assay. The results showed that the 2-morpholinoacetamido derivative exhibited highest FLT3 inhibitory activity among the tested compounds followed by compound I then compound III. Pharmacokinetic assessment disclosed that all the investigated compounds were considered as “drug-like” mols. with promising bioavailability. In the experiment, the researchers used many compounds, for example, 3-(tert-Butyl)isoxazol-5-amine (cas: 59669-59-9Quality Control of 3-(tert-Butyl)isoxazol-5-amine).
3-(tert-Butyl)isoxazol-5-amine (cas: 59669-59-9) belongs to isoxazole derivatives. Isoxazoles present in various natural products and synthetic compounds of biological importance like antibacterial, antagonists, antiinflammatory, analgesics, and also show the applications in functional materials. The most common methods for N–O bond cleavage in isoxazoles are hydrogenation with palladium or platinum catalysts or with Raney Ni. Recent developments have shown that Mo(CO)6 efficiently cleaves the N–O bond in isoxazoles.Quality Control of 3-(tert-Butyl)isoxazol-5-amine
Referemce:
Isoxazole – Wikipedia,
Isoxazole | C3H3NO – PubChem