In general, if the atoms that make up the ring contain heteroatoms, such rings become heterocycles, and organic compounds containing heterocycles are called heterocyclic compounds. An article called A paper-based whole-cell screening assay for directed evolution-driven enzyme engineering, published in 2020-07-31, which mentions a compound: 96-13-9, Name is 2,3-Dibromo-1-propanol, Molecular C3H6Br2O, Formula: C3H6Br2O.
However, the need for a large reagent volume and sophisticated instrumentation hampers its broad implementation. In an attempt to address this problem, here we report a paper-based high-throughput screening approach that should find broad application in generating desired enzymes. As an example case, the dehalogenation reaction of the halohydrin dehalogenase was adopted for assay development. In addition to visual detection, quant. measurements were performed by measuring the color intensity of an image that was photographed by a smartphone and processed using ImageJ free software. The proposed method was first validated using a gold standard method and then applied to mutagenesis library screening with reduced consumption of reagents (i.e., = 10μl per assay) and a shorter assay time. We identified two active mutants (P135A and G137A) with improved activities toward four tested substrates. The assay not only consumes less reagents but also eliminates the need for expensive instrumentation. The proposed method demonstrates the potential of paper-based whole-cell screening coupled with digital image colorimetry as a promising approach for the discovery of industrially important enzymes. Key Points · A frugal method was developed for directed enzyme evolution. · Mutagenesis libraries were successfully screened on a paper platform. · Smartphone imaging was efficiently used to measure enzyme activities.
After consulting a lot of data, we found that this compound(96-13-9)Formula: C3H6Br2O can be used in many types of reactions. And in most cases, this compound has more advantages.
Reference:
Isoxazole – Wikipedia,
Isoxazole | C3H3NO – PubChem