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An environmentally friendly method for the synthesis of lactams and formamides through the biocatalytic alpha-oxidation of amines was developed by employing Pseudomonas plecoglossicida ZMU-T04 as a biocatalyst. In this biocatalytic process, the alpha-oxidation of cyclic amines and N-methylanilines proceeded smoothly to give the corresponding amides in low to high yields. Furthermore, it was demonstrated that synthetic 3,4-dihydroquinolin-2(1H)-one could be used as a key precursor of antidepressant bioactive molecules. The mechanism of this biocatalytic alpha-oxidation process was investigated by isotope- labeling experiments.
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Reference:
Isoxazole – Wikipedia,
Isoxazole | C3H3NO – PubChem